Pages

Showing posts with label transpiration. Show all posts
Showing posts with label transpiration. Show all posts

Wednesday, June 17, 2009

THE GRAVITY OF LIFE Theory by Andrew K Fletcher


Photo courtesy of PDphoto.


Short Introduction for the Association of Science In Schools.


THE GRAVITY OF LIFE by Andrew K Fletcher

Introduction: All life on earth developed with one thing in common; Earth!The constant forces are gravity, and the energy from the sun.

The most abundant resources are minerals and water.

Plants and animals alike, all depend on the properties of water for transporting minerals and nutrients. Because life is based on water, in that everything alive started from a few drops, life must have evolved by finding the easiest and most direct pathway, after all liquids are very good at finding the most direct route possible. Yet, at first glance, everywhere one looks life appears to have chosen the least likely of paths, if it is trying to overcome the effects of gravity. Would trees, with species like the giant Californian redwoods (sequoia sempervirens) , towering over a hundred metres high have chosen a vertical direction? How then have plants and animals harnessed the constant pull of gravity in order to thrive and grow?

On a summer day a large oak tree may take up a hundred gallons of water or more, enriched with minerals and nutrients from the soil. At first glance it is doing so against the pull of gravity, producing flow rates, which cannot be explained or shown by working models based on osmosis, capillary action or root pressure. So how are trees doing it?

Explanation:

Over 95% of the waters drawn in at the roots of a tree evaporate into the surrounding air through the leaves by transpiration. The evaporated moisture contains no minerals. However, the water remaining inside the tree contains a variety of mineral salts dissolved from the soil, together with sugars produced by the tree. The transpired water results in a concentration of salts and sugars within the leaves. Concentrating a liquid, (sap), which contains substances that are heavier than water, must result in the production of a heavier solution than the pre-transpired liquid. Because of the resulting imbalance in density the heavier solution is drawn towards the base of the tree, due to the effect of gravity (maple syrup, latex and amber are evidence for this). Downward flowing sap occurs predominantly within the phloem vessels. When an excess of concentrated liquid is produced during favourable weather conditions, the downward flowing sap forms new tubes from the cambium, as it is forced down by gravity, in a continual cycle of growth.
In hard woods, sap flows from cell to cell through openings or perforations, in the membrane between abutting vessels.
In soft-woods, the sap flow controls movable valves, or pits - (thin areas), in the walls of conducting tracheids. Concentrated pulses of sap may eventually be found to be present in some xylem vessels, as gravity inevitably finds the most direct route, with the least resistance, to the ground.
But for every action there must always be a reaction, and the reaction in this case is that the downward flowing liquid behaves exactly like a plunger in a syringe. As it flows down it causes the entire contents of connected tubes filled with the less dense liquid to be drawn up.
Here we have a simple power source, which is driven purely by evaporation, posture and gravity.
The forces produced by this phenomenon are easy to demonstrate in simple tubular experiments. The main forces are produced at the head and tail of the falling solutions. The head produces a positive force, or pressure, and the tail produces a negative pressure. I believe that the positive force within the mineral laden sap is responsible for the formation of the tubular structures found in timber. The positive force prevents tubes from closing.
As more sap flows through the same pathways, some of the sap is used to strengthen the tubes which will eventually become strong enough to resist the negative pressures. The tree transports the dilute solution of water and minerals to the leaves using these tubes. Thereafter becoming what we call the xylem vessels.
As the concentrated liquid falls towards the ground, minerals are locked away as timber, while the mineral laden liquid arriving at the roots is inevitably re-diluted by the dilute solution drawn from the soil. The imbalance in the liquid is corrected as it becomes lighter or less dense than the downward flowing sap and begins its journey back to the leaves, where the process continues, providing the tree with a constant supply of water and nutrients.
In the autumn, when the leaves have fallen, the circulation is altered as a greater positive pressure is exerted towards the roots, because transpiration has ceased and therefore fluids flowing towards the top of the tree would be compromised. At this time of the year root growth would be most productive.
As fluid channels begin to offer resistance, the sap must find alternative routes. The new directions may be vertical or horizontal, but always in the path of least resistance. Eventually tubes become redundant and new tubes are formed. Fluids of different specific gravity have been observed to flow in both directions, simultaneously while in the same tube. In fact this ‘transpiring gravitational flow system’ is able to operate without tubes and has been attributed to causing the oceans to circulate (Atlantic conveyor system).
Early attempts at lifting water: The story goes that the reigning Grand Duke of Tuscany had ordered a well to be dug to supply the ducal palace with water. The workmen came upon water at a depth of 40 feet, and the next step was to pump it up. A vacuum lift pump was erected over the well, and a pipe let down to the water, but the water was found to rise to a height of 33 feet and no more, in spite of the most careful overhauling of the pump mechanism. It was at this stage that Galileo was consulted. While the famous philosopher was unable to offer a solution, he at least indicated the problem. Here above the 33 feet of water was seven feet of vacuum. The limit for raising water by suction in a tube appeared to be thirty-three feet.
Why should there be this limit when trees are observed to ignore it?
By introducing a loop of tubing, instead of a single tube, to simulate the internal structure of plants and trees, and suspending it by the centre, the problem of raising water above the 33 feet limit is solved. The reason a loop of tubing succeeds where a single tube fails is because the cohesive bond of water molecules is far stronger than the adhesive qualities of water observed in Galileo’s lift-pump problem. Using a loop of tubing enables water molecules to bond to each other in an unbroken chain. It helps to picture the unbroken loop of water as a cord instead of a liquid, supported by a pulley in the centre with tension applied to both ends.
The columns of water held in both sides of the tube exert a downward force due to the weight of the water contained in the tube. This force causes the water molecules in the tube to be stretched, causing the water to behave like an elastic band. In order to demonstrate this affect on water molecules I repeated the experiment shown in figure 1 without the added saline solution, the two open ends of the tube at ground level were removed from the demijohns, exposing them to the air.
Though the tube contained water, it did not flow from either side of the tube. In fact the opposite effect was observed; the water level in both sides of the tube immediately rose to a new level about half a metre from the ends of the tube. Even more surprising the water columns stayed there suspended by the cohesion between the water molecules.
In order to try to upset the balance I then blew up one side of the tube, causing the water level on that side to rise. I then released the pressure and the water returned to the same equal level. This observation offers an exciting explanation to the problem of explaining why water does not pour from the wound when a tree is felled.
However, the present laws of physics state that water cannot exist in its liquid form below 4.6 torr, yet the water remains in the tube. Only when the tube is lowered, or if a bubble appears at the top of the loop of tubing does the water flow out from the open ends.

THE BRIXHAM CLIFF EXPERIMENT

This experiment successfully demonstrated fluid transport to a height, which exceeds the current accepted limit of 10 metres and how this applies to the way that trees draw water to their leaves.
APPARATUS
48 metre single length of clear nylon tubing, 6.35 mm inside diameter x 9.5 mm outside diameter (type used to draw ales in the brewery trade), two clear glass demijohns, a large tray, 50 mils of concentrated salt solution with added red food dye, 50ml syringe minus the needle, sufficient degassed or previously boiled and cooled water to fill the tubing, the demijohns, and for adequate top ups. Adequate nylon cord to hoist the tubing and pulley to the desired height, a small pulley and adhesive cello-tape.
Method
The two bottles were filled to the brim with the water and placed in a suitable tray to catch any displaced water. The length of tubing was half filled with the water by siphoning. This was achieved by submerging one end of the tube in the water filled demijohn placed on a table. When the water reached the centre of the loop, the open end of the tube was capped with a thumb. The end of the tube in the demijohn was removed and the 50 mils of coloured salt water was introduced via the large syringe. The demijohn was then re-filled to the brim and the tube was re-submerged, making sure that no bubbles were introduced by adjusting the height of the unfilled side of the tube. By removing the thumb, the remaining length of tube was filled and again capped, making sure that no air was trapped inside the tube. At this point the demijohns were, refilled. The capped end of the tube was then inserted into the other water filled demijohn and both ends secured at an equal level, with cello-tape, again making sure that no air was allowed to enter the tube.
A length of the nylon cord equal to that of the length of tubing used was passed through the pulley, provided a safe ground level means to hoist the loop of tubing to the desired height. The pulley and the main nylon cord was hoisted to the desired height and secured at the top of the cliff on a separate length of cord. Adhesive cello-tape was wrapped heavily around the two sides of the loop of tubing 15cm from its centre to secure one knotted end of the main nylon cord, which ran through the pulley for the purpose of lifting the tube, taking care not to reduce the tubes diameter. The cello-tape was used to bind the cord to the tube.
Coloured insulation tape was used to secure both sides of the tube together providing an excellent ascent measurement when placed at one-metre intervals.
The Original Brixham Cliff Experiment on Youtube:

The centre of the tube was then gently hoisted, taking care to keep the ascent as smooth as possible. As the tube was raised the salt solution began to fall, due to the influence of gravity; this caused one of the demijohns to start overflowing indicating a positive pressure, while the second demijohn began to lose water at the same rate indicating a negative pressure. The emptying demijohn received frequent top ups, until the salt solution arrived at the overflowing demijohn and the flow stopped.
Conclusion
The fifty mils of salt solution caused the water in the tubes to circulate. The amount of water displaced and collected in the tray represents approximately the volume of water held in one side of the tube. Which meant that the fifty mils of salt solution had lifted water from one demijohn to the height of 24 metres and caused water many times its own weight and volume to rise. (I have used as little as 10 mils of coloured salt solution in the same experiment with a slower rate of decent but with similar displacements of water). Initially the experiments were tested at lower levels of elevation. 24 metres vertical lift was achieved when demonstrating the phenomenon before an audience of journalists and Forestry Commission scientists at the Overgang cliff, Brixham, July 1995. See Press Cutting
Bench demonstration (pictured above) Video of experiment on Youtube

For the purpose of demonstrating this phenomenon use a scaled down two metre high version of Fig 1. Substituting the demijohns for small narrow necked bottles. The type of tubing used to oxygenate aquariums is ideal for this purpose. A two-mil syringe minus needle, filled with coloured salt solution, connected to a T piece via a short length of tube, may be added close to the centre of the elevated tube to introduce salt solution intermittently while the tube is elevated, providing multiple demonstrations. Furthermore, the tube used in the salt free side of the experiment, (return side), may be of a larger bore size. Soft wall, silicon tubing shows visible signs of distortion when the saline solution is allowed to flow through it. The side containing the saline solution expands while the other side contracts, again indicating the presence of both positive and negative, pressures.
The experiments shown have been repeated using a variety of substitutes for salt solution, such as strong tea solution, fruit juices and milk etc. in order to relate directly to plants and animals. The flow rates achieved using different solutions, produced different rates of flow.
Umbrella Plant Experiment, (cyperus alternifolium)


In order to demonstrate that liquids of higher concentrations move through plants in relation to the constant pull of gravity. Take a freshly cut stem about 15cm long, with leaves intact, from an umbrella plant. Place the cutting upside down, in a glass container of water. After several weeks the umbrella plant starts to grow roots from what was the top of the plant and new stems are produced, as the shoots grow vertically in the normal way. The liquid processes involved within the plant for both root and leaf production, must have travelled from one end of the cut stem to the other. Indicating that gravity has an important influence.
When relating back to trees, the negative pressure, observed in the demijohn with the falling water level, provides us with a clear understanding of the mechanisms involved in drawing water through the roots from the soil. The positive pressures caused by the weight of the column of water held in the tree, plus the additional influence of gravity acting on the concentrated solutions, induced by the loss of moisture at the leaf, provides the roots with sufficient power to penetrate the earth.

Explanation for fluid exuding from a cut stem.

To demonstrate this effect, fill a vertically held open ended u tube with water, Fig 2A, and add a little coloured concentrated salt solution to one side, Fig 2B, the level of the salt solution will drop causing the opposite side to overflow. Imagine the loop of tubing is one of many tubes in the stem of a freshly cut plant or tree with roots in the soil. The overflowing water represents the xylem sap rising under the influence of the positive pressure, generated by gravity acting upon the concentrated sap in the phloem tube.
This is an important observation that gives a clear understanding of why plants and trees continue to grow upwards.

Little or no cross contamination takes place between liquids in the clean-water-side and the coloured saline side of the tube. Fig 2 C, I have left this experiment suspended for five days and it appears to remain stable. Circulation within an enclosed system, Fig 3, eliminates siphon as an explanation, demonstrating that flow occurs inside and would continue to do so if the tube was pressurised.
See video of experiments on Youtube
The thin columns of water in trees are known to snap, making a cracking sound through a stethoscope. Cavitation occurs immediately the bead of water separates. The formation of gas at the uppermost part of the raised loop of tubing, Fig 1, caused both columns of water to fall towards the ground and form a new level of 33 feet. The space above the water columns is a vacuum.
The circulation in trees continues, despite continuous cavitations, which means that they are able to refill or repair the vacuum. The internal part of a tree is a network of veins, or tubes, most of which run vertically. However some tubes run at an angle and some horizontally and provide links to other tubes, which interconnect at random levels. The internal tubular parts of the tree are themselves captivated inside a large tube, which is of course the bark or outer skin.
Water columns within the internal tubes of a tree, are continually stressed under a negative pressure, caused by downward flowing concentrated solutions within the trunk and branches. Cavitation occurs because the long thin columns of water are pulled apart. Immediately the cavitation forms, the internal pressures of that tube switch from a negative pressure to a positive pressure, forcing the more dilute solution in the opposing side of adjoined tubes upwards, Fig 2.B. & Fig 2 C. The downward force causes an increase in the head of water at the top of the tube. It is this increase in the head of water that gives a tree both momentum and direction to follow in its cyclical growth. Furthermore an increase in the positive pressure above the cavitation refills and repairs the vacuum, therefore enabling the tree to continue with water transport, and allowing gas bubbles to percolate upwards and out through the leaves.
This ability of the tree to switch from positive pressure to negative pressure and visa-versa gives us an understanding of the pressures observed in the roots of the tree. The roots being able to drive down through the earth under a positive pressure and expanding forces yet are still able to suck in water under a negative pressure.
Safety
· Students conducting any overhead experiments must observe the same Hard Hat safety regulations imposed on building workers.
· Experiments involving tube elevations higher than classroom levels should always be supervised. The safest area for this kind of experiment to take place is on a spiral staircase. Cliff top experiments are dangerous.
· A nylon line passed through a small pulley block, which has been secured at the desired height, enables the loop of tube to be elevated safely from ground level.
· Boiling water is dangerous and should not be handled or moved until it has cooled sufficiently enough to prevent scalding.
END Or Begining?


















How does this fit with Human and animal circulation? Picture the drawing flat on a table. It does not work. Now picture it vertical or even at a slight head up angle and the whole drawing comes to life with a pich of salt added at the top to increase the density and introduce a driving force as it percolates down to the kidneys and in doing so causes the whole drawing to circulate.

Wednesday, June 10, 2009

GCSE Biology DG Mckean (this started my research)

okFluid

"A new scientific truth is not usually presented in a way to convince its opponents. Rather, they die off, and a rising generation is familiarised with the truth from the start."

Max Planck

The following review came from a letter I wrote to the late professor H T Hammel, who was a respected member of the Max Plank Institute. It was an honour to have shared my work and thoughts with Ted over the years.

This was his initial reply.

Within a couple of weeks I received his reply.

INDIANA UNIVERSITY SCHOOL OF MEDIICINE date September 6/ 1995

Dear Mr Fletcher:



I received the information you sent me regarding your ideas about fluid transport in trees, in tubing and in the vascular system in humans.


I will study your ideas and comment upon them as soon as possible. A Quick scan of your Brixham experiment prompts me to ask if you conducted this experiment with boiled water without any solute added to the tubing on either side of the central point which you raise 24 meters? I expect that you could raise the tubing to the same height with or without solute in the water. In any case , your experiment confirms that clean water (water that is unbroken water, water that is without a single minute bubble of vapour) can support tension of several hundreds of atmospheres. The record tension obtained experimentally is 270 atmospheres. At 10 degrees C. (c.f. Briggs, L. Limiting negative pressure of water. Journal of Applied Physics 21: 721-722 1950).


I expect even this tension at brake point can be exceeded by careful cleansing of the water, to remove even the most minute region of gas phase. When the water is already broken, as occurs when gas is entrapped on particulate matter in ordinary water, the water will expand around even a single break when tension (negative Pressure) is applied to the water. When you boil the water, prior to applying (2.4-1) ATM negative pressure to the water in the highest point of the tubing, you eliminate some of these breaks in ordinary water. I expect that dissolving NaCl or other solutes in the water will have little or no effect on the way you measure the tensile strength of water.


I am enclosing some reprints that may interest you. Some of these deal with negative pressures we have measured in tall trees, mangroves and desert shrubs. Other reprints deal with how solutes alter water in aqueous solutions and how colloidal solutes (proteins) affect the flux of protein free fluid between plasma in capillaries and interstitial fluid.


Sincerely H.T. Hammel Ph.D.

GCSE Basic Physiology and water transport.



OSMOSIS ?

"I have chosen to relate to the following text book because it is written by a person who like myself is not entirely satisfied by the explanations put forward in the relevant subjects".


Figure C’s results raise the questions; What is osmosis and how are its qualities explained in the text books.

For the currently accepted view of osmosis and all other views on water transport I will refer to one of the standard GCSE text books entitled GCSE BIOLOGY, D.G. Mackean. ISBN 0-7195-4281-2 first published in 1986.


Page 34 fig 3 Diffusion gradient


Page 36 OSMOSIS


Osmosis is the special name used to describe the diffusion of water across a membrane, from a dilute solution to a more concentrated solution. In biology this usually means the diffusion of water into or out of cells Osmosis is just one special kind of because it is only water molecules and their movement we are considering. Figure 3 showed that molecules will diffuse from a region where there are a lot of them to a region where they are fewer in number; that is from a region of highly concentrated molecules to a region of lower concentration. Pure water has the highest possible concentration of water molecules; it is 100% water molecules, all of them free to move.


Figure 9 shows a concentrated sugar solution, separated from a dilute solution by a membrane, which allows water molecules to pass through. The dilute solution, in effect contains more water molecules than the concentrated solution. As a result of this difference in concentration, water molecules will diffuse from the dilute to the concentrated solution. The level of the concentrated solution will rise or, if it is confined to an enclosed space, its pressure will increase. The membrane separating the two solutions is often called selectively permeable or semi-permeable because it appears as if water molecules can pass through it more easily than sugar molecules can.


Osmosis then is the passage of water across a selectively permeable membrane from a dilute solution to a concentrated solution.

This is all you need to know in order to understand the effects of osmosis in living organisms, But a more complete explanation is given below.

ALTERNATIVE EXPLANATION FOR OSMOSIS


The current text book explanation for osmosis appears to have ignored the effects of gravity on liquids. The constant pull of gravity acts differently on concentrated solutions than dilute solutions i.e. The concentrated solution is heavier than the dilute solution and will always settle at the bottom of a reservoir or in this case a vessel.


To see this clearly, picture Fig 9 without the membrane; the result would be that the concentrated solution would sink and the dilute solution would rise. This effect will not stop because of the membrane. The concentrated solution will still cause the dilute solution to rise as we have seen earlier; and as the concentrated solution moves into the opposite side containing the dilute solution, the dilute solution is dragged through the membrane in a circular motion. For every action there must be a reaction. In order to prove this point add a little dye to the sugar solution and watch the exchange between the liquids.


"When the effect that gravity exerts on concentrated solutions is added to the equation of water transport and osmosis, it gives us a very clear understanding of the driving mechanisms involved".



Chapter 7 Transport in plants


page 71



The main force which draws water from the soil and through the plant is caused by a process called transpiration. Water evaporates from the leaves and causes a kind of ‘suction ‘ which pulls water up the stem. The water travels up the vessels in the vascular bundles and this flow of water is called the transpiration stream. The water vapour passes by diffusion through the air spaces in the mesophyll and out of the stomata. It is this loss of water vapour from the leaves which is called transpiration. The cell walls which are losing water in this way replace it by drawing water from the nearest vein. Most of this water travels along the cell walls without actually going inside the cells. Thousands of leaf cells are evaporating water like this and drawing water to replace it from the xylem vessels in the veins. As a result , water is pulled through the xylem vessels and up the stem from the roots. This transpiration pull is strong enough to draw up water 50 metres or more in trees.


Page 72


Most of this water evaporates from the leaves; only a tiny fraction is retained for photosynthesis and to maintain the turgor of the cells. The advantage to the plant of this excessive evaporation is not clear.


A rapid water flow may be needed to obtain sufficient mineral salts, which are in very dilute solution in the soil. Evaporation may also help to cool the leaf when exposed to intense sunlight.


Against the first possibility it has to be pointed out that, in some cases, an increased transpiration rate does not increase the uptake of minerals.


Many biologists regard transpiration as an inevitable consequence of photosynthesis, in order to photosynthesise, a leaf has to take in carbon dioxide from the air. The pathway that lets carbon dioxide in will also let water vapour out whether the plant needs to lose water or not. In all probability, plants have to maintain a careful balance between the optimum intake of carbon dioxide and a damaging loss of water.


Page 73


Humidity if the air is very humid, i.e. contains a great deal of water vapour, it can accept very little more from the plants and so transpiration slows down. In dry air, the diffusion of water vapour from the leaf to the atmosphere will be rapid. ( " I will deal with this point later on because it is very important and has implications for human health ") Air Movements: In still air, the region round a transpiring leaf will become saturated with water vapour so that no more can escape from the leaf. In these conditions, transpiration slows down. In moving air the water vapour will be swept away from the leaf as fast as it diffuses out. This will Speed up the transpiration. Furthermore, when the sun shines on the leaves, they will absorb heat as well as light. This warms them up and increases the rate of evaporation.


Page 73 continued Water movement in the xylem


You may have learned in physics that you cannot draw water up by suction to a height of more than about ten metres. Many trees are taller than this yet they can draw up water effectively. The explanation offered is that, in long vertical columns of water in very thin tubes, the attractive forces between the water molecules are greater than the forces trying to separate them. So in effect the transpiration stream is pulling up thin threads of water which resist the tendency to break.


There are still problems however, it is likely that the water columns in some of the vessels do have air breaks in them and yet the total water flow is not affected. The evidence all points to the non-living xylem vessels as the main route by which water passes from the soil to the leaves.


"This statement suggests that the long thin tubes of the tree ,are used for water transport, which are none-living , therefore must represent the tubes used in my experiments at Brixham."



Page 74


Root Pressure



In Experiment 8 on page 79 it is demonstrated that liquid may be forced up a stem by root pressure from the root system. The usual explanation for this is that the cell sap in the root hairs is more concentrated than the


soil water and so water enters by osmosis (see page 36). The water passes from cell to cell by osmosis and is finally forced into the xylem vessels in the centre of the root and up the stem.


This is rather an elaborate model from very little evidence. For example, a gradient of falling osmotic potentials from the outside to the inside of a root has not been demonstrated. However, there is some supporting evidence for the movement of water as a result of root pressure.


root pressures of 1-2 atmospheres have been recorded, and these would support columns of water 10 or 20 metres high. Some workers claim pressures of up to eight atmospheres (i.e. 80 metres of water)


" A column of water 80 metres high would undoubtedly cause water pressures of eight atmospheres at the roots. However It is very difficult to see how a root could generate 8 atmospheres of pressure."


However, root pressure seems to occur mainly in the young herbaceous (i.e. non-woody) plants or in woody plants early in the growing season and though in many species it must contribute to water movements in the stem. The observed rates of flow are too fast to be explained by root pressure alone.


Transport of salts


The liquid which travels in the xylem is not, in fact pure water. It is a very dilute solution, containing from 0.1to1.0% dissolved solids, mostly amino acids, other organic acids and mineral salts. The organic acids are made in the roots; the mineral salts come from the soil. The faster the flow in the transpiration stream, the more dilute is the xylem sap. Experimental evidence suggests that salts are carried from the soil to the leaves mainly in the xylem vessels.


Transport of food

The xylem sap is always a very dilute solution, but the Phloem sap may contain up to 25 per cent of dissolved solids, The bulk of which consists of sucrose and amino acids.

There is a good deal of evidence to support the view that sucrose amino acids and may other substances are transported in the phloem. The movement of water and salts in the xylem is always upwards, from the soil to the leaf. But in the phloem the sap may be travelling up or down the stem. The carbohydrates made in the leaf during photosynthesis are converted to sucrose and carried out of the leaf to the stem. From here the sucrose may pass upwards to growing buds and fruits or downwards to the roots and storage organs. All parts of a plant which cannot photosynthesise will need a supply of nutrients bought by the phloem. It is possible for substances to be travelling upwards and downwards at the same time in the phloem.


"note the dual flow has been observed in experiments with concentrated solution and water filled tubes."

Page 74 continued

There is no doubt that substances travel in the sieve tubes of the phloem But the mechanism by which they are moved is not fully understood.


There are several theories, which attempt to explain how sucrose and other solutes are transported in the phloem but none of them is entirely satisfactory.


Page 75


Uptake of water and salts


The water tension developed in the vessels by a rapidly transpiring plant is thought to be sufficient to draw water through the root from the soil. The precise pathway taken by the water is the subject of some debate, but the path of least resistance seems to be in or between the cell walls rather than through the cells.


When transpiration is slow, e.g. at night time or just before bud burst in a deciduous tree, then osmosis may play a more important part in the uptake of water.


One problem for this explanation is that it has not been possible to demonstrate that there is an osmotic gradient across the root cortex which could produce this flow of water from cell to cell. Nevertheless, root pressure developed probably by osmosis can be shown to force water up the root system and into the stem


page 76


The methods by which roots take up salts from the soil are not fully understood. Some salts may be carried in with the water drawn up by transpiration and pass mainly along the cell walls in the root cortex and into the xylem.


It may be that diffusion from a relatively high concentration in the soil to a lower concentration in the root cells accounts for uptake of some individual salts. But it has been shown (a) that salts can be taken from the soil even when their concentration is below that in the roots and (b) that anything which interferes with respiration impairs the uptake of salts. This suggests that active transport (p.35) plays an important part in the uptake of salts.


The thing that becomes clear from reading the established explanations for water transport is that if it were a bucket, very little water would be transported due to the large number of holes in it !